Sintesis Nanopartikel Perak Menggunakan Ekstrak Daun Bidara Ziziphus Spina-Christi Dan Uji Aktivitas Antibakteri Terhadap Bakteri Staphylococcus Aureus
DOI:
https://doi.org/10.61132/obat.v2i1.168Keywords:
Silver Nanoparticles, Ziziphus Spina-Christi, AntibacterialAbstract
Nanotechnology is currently growing rapidly due to its broad application in science and technology. Nanotechnology itself is a science based on nanoparticles, so a more environmentally friendly method is being developed, namely the "green synthesis" method using plant extracts as a bioreductor. Silver nanoparticles have been synthesized using extracts of bidara leaf as a reducing agent. The objective of this research was was to determine the synthesis of silver nanoparticles using bioreductors and their application as antibacterial agents using bidara leaf extract against Staphylococcus aureus bacteria. This research used the green synthesis method to produce silver nanoparticles. The bidara leaf extract used was divided into several concentrations, namely 1 mM, 2 mM, 3 mM and 4 mM. To characterize silver nanoparticles, UV-Vis and PSA spectrophotometers were used. Furthermore, the Kirby-Bauer method was used to test the antibacterial properties of silver nanoparticles against Staphylococcus aureus bacteria. The results of research that has been done show that the formation of silver nanoparticles is characterized by the emergence of maximum absorbance peaks in the wavelength range of 400-450 nm, while the PSA results at concentrations of 3 and 4µm showed nanoparticle sizes of 185 nm. Silver nanoparticles of bidara leaf extract with various concentrations of 1 mM, 2 mM, 3 mM and 4 mM have antibacterial activity that can inhibit the growth of Staphylococcus aureus bacteria with an average inhibition zone diameter of 13.0617 mm, 11.7333 mm, 9.05 mm and 13.19 mm. This shows that silver nanoparticles with bidara leaf extract have antibacterial properties.
References
Adam, dkk. (2022). Pengaruh Penambahan Poli. Pengaruh Penambahan Poli Vinil Alkohol Terhadap Ukuran Dan Kestabilan Nanopartikel Perak Hasil Sintesis Menggunakan Bioreduktor Ekstrak Daun Akasia (Accasia Mangium Wild), 23(1), 50–58.
Asy’syifa, Nurlaeli Siti, Fitrianti Darusman, & M. L. D. (2020). Uji Aktivitas Antibakteri Ekstrak Daun Bidara Arab (Ziziphus Spina- ChristiL.) Terhadap Staphylococcus aureus dan Escherichia coli. Prosiding Farmasi, 6(2).
Din, M. I., & Rehan, R. (2017). Synthesis, Characterization, and Applications of Copper Nanoparticles. Analytical Letters, 50(1), 50–62.
Fabiani, V. A., Silvia, D., Liyana, D., & Akbar, H. (2019). Sintesis Nanopartikel Perak Menggunakan Bioreduktor Ekstrak Daun Pucuk Idat (Cratoxlum glaucum) melalui Iradiasi Microwave serta Uji Aktivitasnya sebagai Antibakteri. Fullerene Journ. Of Chem, 4(2), 96–101.
Faidah, N, I. 2019. Biosintesis Nanopartikel Perak (Agnp) Ekstrak Buah Tin (Ficus Carica L.) Terhadap Aktivitas Antioksidan Dan Toksisitas Larva Artemia salina. Energies, 6(Usu): 1–8.
Hasheminya, S. M., & Dehghannya, J. (2020). Green synthesis and characterization of copper nanoparticles using Eryngium caucasicum Trautv aqueous extracts and its antioxidant and antimicrobial properties. In Particulate Science and Technology (Vol. 38, Issue 8).
Khani, R., Roostaei, B., Bagherzade, G., & M., & M. (2018). Green synthesis of copper nanoparticles by fruit extract of Ziziphus spina-christi (L.) Willd.: Application for adsorption of triphenylmethane dye and antibacterial assay. Journal of Molecular Liquids, 255, 541–549.
Mardhiyani, D., Afriani, M., Farmasi, F., Kesehatan, I., & Abdurrab, U. (2021). Antibacterial Activity Test Of Leaves Bidara (Ziziphus Mauritiana Lam) Ethanolic Extracts Against Staphylococcus Aureus Uji Antibakteri Ekstrak Etanol Daun Bidara (Ziziphus Mauritiana Lam) Terhadap Bakteri Staphylococcus Aureus. Jurnal Proteksi Kesehatan, 10(1), 44–48.
Marfu’ah, N., & Ramadhani, C. A. (2019). Uji Efektivitas Ekstrak Etanol Daun Bidara (Ziziphus spina-christi L.) Terhadap Pertumbuhan Propionibacterium acne (Vol. 3, Issue 1).
Masykuroh, A., & Heny, P. (2022). BIOMA: Jurnal Biologi Makassar (Online) Aktivitas Anti Bakteri Nano Partikel Perak (NPP) Hasil Biosintesis Menggunakan Ekstrak Keladi Sarawak Alocasia macrorrhizos terhadap Staphylococcus aureus dan Escherichia coli Antibacterial Activity Of Silver Nano Particles Biosynthesized Using Alocasia macrorrhizos Extract Against Staphylococcus aureus and Escherichia coli. 7(1), 76–85.
Mauludiyah, E. N., Darusman, F., & Darma, G. C. E. (2020). Skrining Fitokimia Senyawa Metabolit Sekunder dari Simplisia dan Ekstrak Air Daun Bidara Arab (Ziziphus spina-christi L.). Prosiding Farmasi, 1084–1089.
Mutia Windy, Y., Natasya Dilla, K., Claudia, J., & Rakhman Hakim, A. (2022). Characterization And Formulation Of Nanoparticles Extract Of Bundung Plant (Actinoscirpus Grossus) With Variations In Concentration Of Chitosan And Na-TPP Bases Using The Ionic Gelation Method. Karakterisasi Dan Formulasi Nanopartikel Ekstrak Tanaman Bundung , 3, 25–29.
Purnamasari, M. D., Dan, H., & Wijayati, N. (2016). Indonesian Journal of Chemical Science. J. Chem. Sci, 5(2).
Purnaningrum, R. & Eli, R. (2017). Aktivitas Antibakteri Kain Nylon 6,6 Dengan Penambahan Nanopartikel Perak Terhadap Staphylococcus aureus ATCC 25924. Jurnal Kimia Dasar, 6(4).
Sharma, P., Pant, S., Dave, V., Tak, K., Sadhu, V., & & Reddy, K. R. (2019). Green synthesis and characterization of copper nanoparticles by Tinospora cardifolia to produce naturefriendly copper nano-coated fabric and their antimicrobial evaluation. Journal of Microbiological Methods, 160, 107–116.
Sumiati, T., Ratnasari, D., Dwi Mutiani, D., Studi Farmasi Sekolah Tinggi Teknologi Industri dan Farmasi Bogor, P., Program Studi, M. S., & Sekolah Tinggi Teknologi Industri dan Farmasi Bogor, F. (2018). Sintesis Nanopartikel Perak Menggunakan Ekstrak Kulit Bawang Merah (Allium cepa L.) dan Uji Aktivitas Antibakteri Terhadap Bakteri Staphylococcus aureus (Vol. 3, Issue 1).
Suriaman, E. (2017). Skrining Aktivitas Antibakteri Daun Kelor (Moringa oleifera), Daun Bidara Laut (Strychnos ligustrina Blume) dan Amoxicilin Terhadap Bakteri Patogen Staphylococcus aureus. Jurnal Biota 3(1).
Taba, P., Parmitha, N. Y., & Kasim, S. (2019). Sintesis Nanopartikel Perak Menggunakan Ekstrak Daun Salam (Syzygium polyanthum) Sebagai Bioreduktor Dan Uji Aktivitasnya Sebagai Antioksidan Synthesis of Silver Nanoparticles Using Syzygium polyanthum Extract as Bioreductor and the Application as Antioxidant. J. Chem. Res, 7(1), 51–60.
Usman, S., Firawati, F., & Zulkifli, Z. (2021). Efektivitas Ekstrak Daun Bidara (Zizipus Mauritiana L.) pada Kulit Akibat luka Bakar dalam Berbagai Varian Konsentrasi Ekstrak Terhadap Hewan Uji Kelinci (Oryctolagus cuniculus L.). Jurnal Sains Dan Kesehatan, 3(3), 430–436.
Wisnuwardhani, D. (2019). Optimasi Kondisi Sintesis Nanopartikel Tembaga Menggunakan Ekstrak Biji Melinjo (Gnetum gnemon L.). Jurnal Ilmiah Ibnu Sina, 4(2), 452–459.
