Efektivitas Sabun Cuci Tangan Ekstrak Daun Afrika ( Vernonia amygdalina Del.) Terhadap Bakteri Escherichia coli

Panji Ratih Suci; Cikra Ikhda Nur H.S; Anita Candra Ningtyas; Annisa Lailatul Maghfiroh

doi:10.61132/obat.v2i3.364

Authors

Panji Ratih Suci Akademi Farmasi Mitra Sehat Mandiri Sidoarjo
Cikra Ikhda Nur H.S Akademi Farmasi Mitra Sehat Mandiri Sidoarjo
Anita Candra Ningtyas Akademi Farmasi Mitra Sehat Mandiri Sidoarjo
Annisa Lailatul Maghfiroh Akademi Farmasi Mitra Sehat Mandiri Sidoarjo

DOI:

https://doi.org/10.61132/obat.v2i3.364

Keywords:

Bitter leaf, Eshcherichia coli, paper discs

Abstract

African leaf plants (Vernonia amygdalina Del.) is one of tradisional medicine and has pharmacological contain secondary metabolite such as Flavonoids, Saponin, Tanin and Alkaloid. This study aimed to determine the antibacterial activity of African leaf extract (Vernonia amygdalina Del.) in handsoap formulation on the growth of Escherichia coli. African left extract was obtained from maceration process with ethanol and phytochrmical screening was carried out.This study uses concentration of 0,5%, 1% and 2%. Antibacterial activity testing using scratch and disc diffusion methods. Tis study also evaluated the pyshical quality of the preparation which included organoleptic observation, homogeneity test, high foam test and antibacterial activity test. Antibacterial activity testing using scratch and disc disfusion methods. African pyctochemical screening was carried out. The result showed that the percentage of African leaf extract yield was 24,958%. The pH test gets a pH of 10-11 and 5-6 cm foam height test. The highest concentration of positive control of preparation containing alcohol (Dettol) with an average number og 4,72 mm ± 1,45 mm. Liquid soap preparation 1% with a diameter of inhibition of 3,93 mm ± 1,97 mm. The result of this study inhibit the growth of Eshcherichia coli bacteria.

References

Amaliah, Siti.2010.”Hubungan Sanitasi Lingkungan Dan Faktor Budaya Dengan Kejadian Diare Pada Anak Balita di Desa Toriyo Kecamatan Bendosari Kabupaten Sukoharjo”.Prosiding Seminar Nasional Unimus 2010.

Desiyanto, F. A., & Djannah, S. N. (2013). Efektivitas mencuci tangan menggunakan cairan pembersih tangan antiseptik (hand sanitizer) terhadap jumlah angka kuman. Kes Mas: Jurnal Fakultas Kesehatan Masyarakat Universitas Ahmad Daulan, 7(2), 24934.

Kasenda, J. C. (2016). Formulasi dan pengujian aktivitas antibakteri sabun cair ekstrak etanol daun ekor kucing (Acalypha hispida Burm. F) terhadap pertumbuhan bakteri Staphylococcus aureus. Pharmacon, 5(3).

Melliawati, R. (2009). Escherichia coli dalam kehidupan manusia. Bio Trend, 4 (1), 10–14.

Setyani, W., Setyowati, H., & Ayuningtyas, D. (2016). Pemanfaatan ekstrak terstandarisasi daun som jawa (Talinum paniculatum)(Jacq. Gaertn) dalam sediaan krim anti bakteri Staphylococcus aureus. Jurnal Farmasi Sains dan Komunitas, 13(10), 44-51.

Suryati, S., Dillasamola, D., & Rahadiant, F. (2016). Pengaruh Ekstrak Etanol Daun Vernonia amygdalina, Del terhadap Kadar Kreatinin Serum Mencit Putih Jantan. JSFK (Jurnal Sains Farmasi & Klinis), 3(1), 79-83.

Swandiny, G. F., & Kumala, S. Aktivitas Antibakteri menggunakan Metode Difusi Cakram terhadap Ekstrak Etanol 70% Daun Afrika (Vernonia amygdalina Del.). SCIENTIFIC COMMITTEE, 47.

Tjay, T. H., & Rahardja, K. (2007). Obat-obat penting: khasiat, penggunaan dan efek-efek sampingnya. Elex Media Komputindo.

Yulianti, R., Nugraha, D. A., & Nurdianti, L. (2015). Formulasi Sediaan Sabun Mandi Cair Ekstrak Daun Kumis Kucing (Orthosiphon Aristatus (Bl) Miq.). Kartika: Jurnal Ilmiah Farmasi, 3(2), 1-11.