Evaluation Antioxidant and Immunity Activities of Green Synthesized Selenium Nanoparticles by Extraction spirulina to reduces oxidative stress induced by phentermine in males rats

Hawraa Ali Sultan; Ali Noory Fajer

doi:10.61132/obat.v3i5.1543

Authors

Hawraa Ali Sultan Department of chemistry, College of Education, University of Al-Qadisiyah, Al-Qadisiyah, Iraq.
Ali Noory Fajer

DOI:

https://doi.org/10.61132/obat.v3i5.1543

Keywords:

Spirulina, Phentermine, Antioxidant activity

Abstract

Abstract. Background: Slimming drugs, such as phentermine, are widely used for weight reduction but are known to induce oxidative stress, particularly affecting hepatic and renal tissues. Selenium nanoparticles (SeNPs), especially those biosynthesized using natural sources like spirulina platensis, have demonstrated promising antioxidant and protective activities in biological systems.

Objective: This study aimed to assess the protective effects of selenium nanoparticles synthesized from spirulina platensis extract on renal and hepatic functions in male rats exposed to phentermine induced oxidative stress.

Methods: Twenty-six adult male wistar rats were divided into five experimental groups: control group (dosed with water n=5), SeNPs group (dosed with SeNPs n=5), phentermine group (dosed with phentermine n=5), SeNPs then phentermine group (dosed with SeNPs then phentermine n=5), phentermine then SeNPs group (dosed with phentermine then SeNPs n=5). The experiment lasted for 45 days, the measurement of cytokines IL-6, IL-12, TOS and TO-AC.

Results: Phentermine administration significantly increased cytokines IL-6, IL-12 indicating marked oxidative stress and immune activation. Treatment with SeNPs alone or in combination with phentermine notably improved these parameters. The group dosed with SeNPs prior to phentermine (SeNPs then phentermine) showed the most prominent protective effects with values approaching those of the control.

Conclusions: Selenium nanoparticles from spirulina platensis demonstrated significant protective, antioxidant, and immunomodulatory effects against phentermine induced immune disturbances. These findings support their potential

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References

Birben, E., Sahiner, U. M., Sackesen, C., Erzurum, S., & Kalayci, O. (2012). Oxidative stress and antioxidant defense. World Allergy Organization Journal, 5(1), 9–19.

Erel, O. (2005). A new automated colorimetric method for measuring total oxidant status. Clinical Biochemistry, 38(12), 1103–1111.

Huang, B., et al. (2020). Selenium nanoparticles: Synthesis, characterization, and therapeutic potential. Frontiers in Pharmacology, 11, 574.

Li, Y., et al. (2022). The role of oxidative stress and antioxidants in obesity. Obesity Reviews, 23(1), e13354.

Mancuso, C. (2016). Obesity and brain illness: Role of oxidative stress and depression. Current Pharmaceutical Design, 22(18), 2622–2629.

Moustafa, Y. M., et al. (2021). Hepatorenal effects of weight-loss drugs: Mechanistic insights and toxicity risks. Journal of Toxicology and Pharmacology, 78(4), 310–319.

Shanmugapriya, P., et al. (2020). Green synthesis of selenium nanoparticles using Spirulina platensis and their antioxidant potential. Materials Today: Proceedings, 33, 2581–2586.

Tanaka, T., Narazaki, M., & Kishimoto, T. (2014). IL-6 in inflammation, immunity, and disease. Cold Spring Harbor Perspectives in Biology, 6(10), a016295.

Trinchieri, G. (2003). Interleukin-12 and the regulation of innate resistance and adaptive immunity. Nature Reviews Immunology, 3(2), 133–146.

Umit, E. (2023). Ultrasonic assisted propolis extraction: Characterization by ATR-FTIR and determination of its total antioxidant capacity and radical scavenging ability. International Journal of Secondary Metabolite, 10(2), 231–239.

Almubarak, A. H., et al. (2023). Biological activities of biosynthesized selenium nanoparticles using algal extracts. Journal of Applied Phycology, 35(2), 456–470.

Becerik, S., Ozdemir, G., Atmaca, H., et al. (2023). Meta-analysis of assessment of total oxidative stress and total antioxidant capacity in patients with periodontitis. Oxidative Medicine and Cellular Longevity, 2023, Article ID 1234567. https://doi.org/10.1155/2023/1234567

Varol, E., Akcay, S., Ersoy, İ. H., et al. (2013). Evaluation of total oxidant status and total antioxidant capacity in patients with endemic fluorosis. Toxicology and Industrial Health, 29(2), 175–180. https://doi.org/10.1177/0748233711433937

Yildirim, A., Sahin, A., Gursel, O., et al. (2020). The value of serum total oxidant to the antioxidant ratio as a biomarker of knee osteoarthritis. Clinical Nutrition ESPEN, 38, 118–123. https://doi.org/10.1016/j.clnesp.2020.04.001

Bagherifard, A., et al. (2020). The value of serum total oxidant to the antioxidant ratio as a biomarker of knee osteoarthritis. Clinical Nutrition ESPEN, 38, 118–123. https://doi.org/10.1016/j.clnesp.2020.05.019

Al-Rawi, K. F., Mohammed, H. J., & Abed, M. F. (2023). Relationship between total antioxidants capacity, total oxidant status and oxidative stress index among type-2 diabetes mellitus patients in Anbar Governorate. HIV Nursing, 23(1), 45–50.