The role of sperm DNA fragmentation on male infertility in  Al kut city

Othman Hashem Mohammed; Rania Sabri Hasan; Ghassan Jabbar Khalaf; Haidar Hussein Essa; Luma Hikmat Kareem; Wisam Basim Al- Tmemy; Zeena Mohammed Abdul Hassan

doi:10.61132/obat.v2i6.880

Authors

Othman Hashem Mohammed Embryology - kut University College , Wasit –IRAQ
Rania Sabri Hasan Microbiology- Central Technical University-Technical Institute-Kut
Ghassan Jabbar Khalaf Parasitology- kut University College
Haidar Hussein Essa Microbiology - kut University College
Luma Hikmat Kareem Luma Hikmat Kareem
Wisam Basim Al- Tmemy Wisam Basim Al-Tmemy
Zeena Mohammed Abdul Hassan Molecular Biology- kut University College , Wasit –IRAQ

DOI:

https://doi.org/10.61132/obat.v2i6.880

Keywords:

DFI, SCD , IVF, LH, FSH

Abstract

Infertility is one of the most common diseases in the world in last period of all types,
whether it is due to (asthenospermia,oligoasthenospermia,teratospermia,hormonal imbalance).
Study aims to reveal negative effect of sperm DNA fragmentation on fertilization, this study
was conducted in the Al-Jazeera Specialized Laboratory in the city of Kut for the period
between (January to November from 2024), two hundred A person suffering from infertility.
The semen was examined for all of them, they were divided into eighty of them being normal
(the control group) and one hundred and twenty suffering from infertility (asthenospermia)
hormonal test and sperm DNA fragmentation test (by Kit methods) was performed for everyone
, this study showed Following, we obtained higher proportions of sperm DNA fragmented
infertile Which had an abnormal shape and low motility. The results are similar to those of
other studies, which found that the DFI in infertile group was significantly higher than that in
fertile group (34.53 ± 4.6 % compared to11.91 ± 4.0 %) and mean sperm concentration in
fertile group was significantly higher compared to infertile group (83.22 ± 14.9 /ml compared
to11.67 ± 10.7 /ml) from this study. Through this study, the DFI test they can be used for
discrimination and between the infertile male and normal male . Male men with sperm (DFI ≥
25.1% ) had a (2.52 ) times higher risk of infertility compared to males with sperm (DFI <
25.1%). This study shows that there is a negative relationship between (spem DNA
fragmentation , sperm motility and formation) in infertile males. In addition, it can be concluded

that sperm DNA fragmentation is very useful in selecting and choosing suitable sperm to be
used in many techniques, including assisted reproductive techniques. It can also be concluded
that the results of sperm DNA fragmentation analysis are encouraging and useful and can be
used for diagnostic purposes in determining male infertility

Downloads

Download data is not yet available.

References

- Gurunath, S; Pandian, Z; Anderson , R; et al. (2010):Defining infertility-a systematic review of prevalence studies; (5): 575-576.

American Society for Reproductive Medicine (ASRM).(2011):Infertility an overview. A Guide for Patients. (suppl); S(1):60-162.

American Society for Reproductive Medicine (ASRM) .(2012):A Practice Committee Educational Bulletin. Effectiveness and Treatment for Unexplained Infertility. (Suppl);S(2):121-124.

Gunby, B; Libras, S; Terry, K; et al.(2010): Assisted reproductive technologies (ART); (95): 35- 39.

O’Flynn O’Brien, K; Varghese, A.C .(2012): The genetic causes of male factor infertility; (93): 8-12.

Matschke, H.M; Lipshultz ,L.I.(2011): Male factor infertility; (3):689-696.

Kolasa, A; Misiakiewicz, K; Marchlewicz, M; et al.(2009): The generation of spermatogonial stems cells and spermatogonia in mammals; (7): 78-81.

Hermo, L; Pelletier, R.M; Cyr, D.G;et al.(2012): Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells. Part 1: background to spermatogenesis, spermatogonia, and spermatocytes; (4): 241-278.

Hermo, L, Pelletier, R.M; Cyr, D.G; et al.(2012): Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells. Part 2: changes in spermatid organelles associated with development of spermatozoa; (5): 279-319.

Oxenham, S.K; Barratt, C.L.; Kay, V; et al.(2010): The human spermatozoa—a stripped down but refined machine; (8): 63-67.

Antonucc, N; Stronati, A;Manes, S; et al.(2012): Setup of a novel in vitro sperm head decondensation protocol for a rapid flow cytometric measurement of the DNA content; (25): 24-26.

Mclachan,R.I; Wellford, N.G.(2009): Hormonal control of spermatogenesis; (6):95-101.

Cornwall, G.A.(2011): New insights into epididymal biology and function; (2): 213-227.

Aitken, R.J. (2013):Sperm function tests and fertility;(69): 75- 84.

Menkveld, R; El- Garem, Y; Schill, W;et al.(2013): Relation ship between acrosomal function and sperm morphology; (20):432-438.

Nallella, K.P; Sharma, R.K; Aziz, N; et al.(2014): Significance of sperm characteristics in the evaluation of male infertility; (85): 629–633.

Cooper, T.G; Noonan, E; Eckardstein, S; et al.(2011): World Health Organization reference values for human semen characteristics; (3): 231–239.

Morales, P; Katz, F; Oversireet, J. W; et al. (2012):The Relationship between the Motility and Morphology of Spermatozoa in Human Semen; (9):241-247.

Zanetti, N; Mayorga, L.S.(2013): Acrosomal swelling and membrane docking are required for hybrid vesicle formation during the human sperm acrosome reaction; (2): 396-405.

Adashi, E.Y; Hsueh, A.J.(2014): Direct Inhibition of Testicular Androgen Biosynthesis Revals Anti gonadal Activity of Neuro hypophyseal Hormones Nature; (2):650-652.

Vermeulen, A; Kaufman, J.M.(2013): Ageing of Hypothalamo-pituitary- Testicular Axis in Men; (43):25-30.

Cosigning, P.G ; Somigliana,R.(2010): Effect of GnRH antagonists in FSH mildly stimulated intrauterine insemination cycles: a multi centre randomized trial; (22):500- 505.

Holdswerth, S;Aitkinsg, R.S.(2013): The pituitary-Testicular Axis in Men with Chronic Renal Failar; (92):1245-1249.

Suneet, M; Lakhbir, D; Sanjeev, S;et al. (2009):Infertility.Obstetrics and Gynology;(()24-29.

Garside, D.A; Harvey, P.W.(2012):Endocrine toxicology of the male reproductive system;(2):29-32.

Aiman, J; Mcacey, M;Harms, L.(2013): Serum in seminal plasma prolactin constriction in men with normospermia, oligospermia or azoospermia; (49): 133-137.

Zipf ,W.B; Payne, A.H. (2016):Prolactin, growth hormone, luteinizing hormone receptors.Endocrinology; (103):295-297.

Bhasin, S; Buckwalter, J.G.(2012): Testosterone supplementation in older men; (22): 718-721.

Adamopoulos, D.A; Lawrence, D.M; Vassilopoulos, P; et al.(2010): Pituitary Testicular Inter relationships in Mumps Orchitis and Other Viral Infection; (2): 34-38.

Pandiyan,N.(2011):The Hormonal assessment of the infertile; (83):738-739.