The Impact of the adeE and adeY Genes on the Antibiotic Resistance  Mechanisms of Acinetobacter baumannii

Rawaa M. Mohammed

doi:10.61132/obat.v3i3.1286

Authors

Rawaa M. Mohammed Ph.D .Microbilogy, Nursing College, Al-Mustaqbal University, Iraq, 51001

DOI:

https://doi.org/10.61132/obat.v3i3.1286

Keywords:

isolates, Acinetobacter, biofilm

Abstract

Current study identified Acinetobacter baumannii isolates from burn wound samples using biochemical and
molecular techniques focusing on the rpoB gene. All isolates showed variable virulence factors, including
biofilm formation (100%), gelatinase activity (90%), and mannose-resistant hemagglutination (72%). Antibiotic
susceptibility testing revealed high resistance rates to Rifampin (96%), Tetracycline (92%), and third-
generation cephalosporins such as Cefotaxime (73%) and Ceftriaxone (81%). Lower resistance rates were
observed for Levofloxacin (31%) and Gatifloxacin (27%), suggesting potential effectiveness.
PCR analysis confirmed the presence of AdeE and AdeY efflux pump genes in several isolates. However,
statistical analysis, including Chi-square/Fisher’s tests and t-tests, demonstrated no significant correlation
between the presence of these genes and antibiotic resistance patterns (p > 0.05). This indicates that while
efflux pump genes are present, their presence alone does not predict antibiotic resistance in the tested isolates.
The findings highlight the complex mechanisms of resistance in A. baumannii and the need for continued
surveillance and molecular diagnostics to guide effective infection control.

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